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KARYA TULIS ILMIAH UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN BUAH DELIMA (Punica granatum L.) TERHADAP BAKTERI Salmonella typhi
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ABSTRAK
Salmonella typhi merupakan kuman pathogen penyebab demam tifoid. Salah satu obat tradisional yang digunakan berasal dari tanaman delima (Punica granatum L.) digunakan sebagai obat oleh masyarakat di Indonesia. Tujuan : Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun buah delima (Punica granatum L.) konsentrasi 40% dan 80% terhadap bakteri Salmonella typhi. Metode : Penelitian ini merupakan eksperimental laboratorium. Metode pengujian yang digunakan yaitu metode difusi kertas cakram (paper disc) dan metode tebar (Spread plate method) untuk mengetahui aktivitas antibakteri ekstrak etanol daun buah delima. Hasil : Hasil uji daya hambat antibakteri ekstrak daun etanol buah delima (Punica granatum L.) mempunyai rata-rata diameter zona hambat secara berturut-turut pada konsentrasi 40% rata-rata 6,63 mm (kategori sedang) dengan standar deviasi 0,46, konsentrasi 80% rata-rata 10,90 mm (kategori kuat) dengan standar deviasi 0,34, kontrol positif (Chloramfenicol ) rata-rata 19,87 mm (kategori kuat) dengan standar deviasi 0,49 dan kontrol negatif (Aquadest) menunjukkan tidak ada zona hambat dalam pertumbuhan bakteri. Kesimpulan : Berdasarkan uji ANOVA pada tabel Test Statistisc menunjukkan nilai signifikasi 0,00 yang berarti p< 0,05 sehingga dapat disimpulkan bahwa H0 ditolak, Ekstrak etanol daun buah delima (Punica granatum L.) mempunyai perbedaan perlakuan dalam menghambat bakteri Salmonella tyhpi dengan konsentrasi 40% dan 80% yang dibandingkan kontrol positif (Chloramfenicol) dan kontrol negatif (aquadest).
ABSTRACT
Salmonella typhi is a pathogen that causes typhoid fever. One of the traditional medicines used comes from the pomegranate plant (Punica granatum L.) which is used as medicine by people in Indonesia. Objective: This study aimed to determine the antibacterial activity of the ethanolic extract of pomegranate leaves (Punica granatum L.) at concentrations of 40% and 80% against Salmonella typhi bacteria. Methods : This research is an experimental laboratory. The test method used was the paper disk diffusion method and the spread plate method to determine the antibacterial activity of the ethanolic extract of pomegranate leaves. Results: The results of the antibacterial inhibition test of the ethanolic leaf extract of pomegranate (Punica granatum L.) had an average inhibition zone diameter of 40%, an average of 6.63 mm (medium category) with a standard deviation of 0.46, 80% concentration average 10.90 mm (strong category) with a standard deviation of 0.34, positive control (Chloramfenicol) an average of 19.87 mm (strong category) with a standard deviation of 0.49 and negative control (Aquadest) showed no inhibition zone in bacterial growth. Conclusion: Based on the ANOVA test in the Statistisc Test table shows a significance value of 0.00 which means p < 0.05 so it can be concluded that H0 is rejected, Pomegranate leaf ethanol extract (Punica granatum L.) has different treatments in inhibiting Salmonella typhi bacteria with a concentration of 40% and 80% compared to positive control (Chloramfenicol) and negative control (aquadest).
Detail Information
| Item Type |
KARYA TULIS ILMIAH
|
|---|---|
| Penulis |
Fibria Atik Prihatini - Personal Name
|
| Student ID | |
| Dosen Pembimbing |
Panji Ratih Suci, S.Farm., M.Farm - - Dosen Pembimbing 1
|
| Penguji |
Sih Wahyuni Raharjeng, M.Si - - Ketua Penguji
Apt. Ismu Dwi Supangkat, S.Farm - - Penguji 1 Panji Ratih Suci, S.Farm., M.Farm - - Penguji 2 |
| Kode Prodi PDDIKTI | |
| Edition |
Published
|
| Departement | |
| Contributor | |
| Language |
Indonesia
|
| Publisher | Akademi Farmasi Mitra Sehat Mandiri Sidoarjo : Krian., 2021 |
| Edition |
Published
|
| Subject(s) | |
| No Panggil |
615.14 FIB u
|
| Copyright | |
| Doi |